ECL Western Blotting Ultimate Sensitivity Substrate (Femto)
ECL Western Blotting Ultimate Sensitivity Substrate is a non-radioactive chemiluminescent system for detecting horseradish peroxidase (HRP) on immunoblots. Read more
Transported at room temperature, stored at 4°C away from light, shelf life is 12 months.
This product contains an enhanced luminol substrate and a stable peroxide system for detecting femtogram amounts of antigen and is suitable for chemiluminescent detection in conventional Western Blot.
KEY FEATURES
Stability Working solution remains stable for up to 24 hours
High Cost Performance Compared with other chemiluminescent substrates of the same grade, the cost performance is quite high.
High Sensitivity VQ4726 enables detection down to the low-femtogram level
Ultra-high sensitivity at real femtogram level
The 1 mg/mL actin-HRP antibody was serially diluted to obtain concentrations of 1000, 500, 250, 125, and 62.5 fg/μL. 1μL of each dilution was spotted onto an NC membrane. After spotting, the membrane was developed with YamayBio VQ4726 for 5 minutes.
YamayBio VQ4726 offers higher femtogram-level sensitivity than Thermo 37071.
Lysate: A431 whole cell lysates MW: GAPDH-36kD
Primary antibody: Anti-GAPDH, dilution at 1:5000 Secondary antibody: Goat anti-Mouse IgG(H+L),HRP conjugated at 1:5000 dilution Exposure time: 10 seconds.
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Certificate of Analysis
Safety Data Sheet
Specifications
| Product Type | Substrate |
| Detection Method | Chemiluminescence |
| Form | Liquid |
| Application | Western Blot |
| Quantity | 100 mL / 500 mL |
| Sensitivity | Femtogram |
| Membrane Compatibility | Nitrocellulose, PVDF |
