Animal Membrane and Cytosol Protein Extraction Kit
This kit provides a streamlined and efficient method for the isolation of membrane and cytosolic proteins from animal cells and tissues. Read more
Upon receipt aliquot and store Cell Permeabilization Buffer and Solubilization Buffer at -20°C to avoid freeze/thaws. Store Cell Wash Solution at 4°C. Product is shipped on ice.
This kit provides a streamlined and efficient method for the isolation of membrane and cytosolic proteins from animal cells and tissues. The membrane protein fraction includes plasma membrane proteins as well as proteins associated with mitochondrial, endoplasmic reticulum, and Golgi membranes.
This kit uses a new and highly efficient detergent formulation. First, a mild detergent is used to permeabilize cells and release soluble cytoplasmic proteins. Then, another detergent is used to dissolve membrane proteins. The extraction efficiency depends on the number of times the integral membrane protein(s) span the lipid bilayer. For proteins with at least one or two transmembrane domains, the extraction efficiency is usually as high as 90%. The cross-contamination of cytoplasmic proteins into the membrane fraction is usually less than 10%. Selective detergent extraction eliminates the need for phase separation based on hydrophobicity, allowing for better reproducibility and higher throughput.
KEY FEATURES
High Yield Up to 0.4-0.6 mg of membrane protein can be extracted from 5×10^6 cells, delivering 30-50% higher yields compared to leading competitor products.
High Purity Cross-contamination is maintained below 10%, ensuring minimal cytosolic protein carryover in the membrane fraction.
Simplified Workflow No homogenization, freeze–thaw cycles, or complex pre-treatment steps are required.
Board Compatibility Suitable for membrane protein extraction from animal cells and tissues. Extracted proteins are compatible with BCA protein quantification, SDS-PAGE, Western blot, and immunoprecipitation.
Fast Speed Membrane protein extraction can be completed within about 90 minutes.
Outperforms Competitors with Higher Target Protein Yield
Figure 1. Four membrane proteins (monomer molecular weights: 70.6 kDa, 78.8 kDa,103.6 kDa, and 39.8 kDa) were overexpressed in HEK293F cells. Equal cell numbers (5×10^6) were processed using Thermo 89842 and YamayBio BF7475 kits. Western blot detection was performed using an anti-Strep tag II antibody. Lysate loading: 10 µL per lane.
High-Efficiency Membrane Isolation with Low Cytosolic Cross-Contamination
Figure 2. Membrane (M) and cytosolic (C) fractions were isolated from HEK293F cells using Thermo 89842 and YamayBio BF7475 kits. Western blot analysis was performed using an anti -HSP90 antibody. Lysate loading: 10 µL per lane.
30–50% Higher Membrane Protein Yield Across Multiple Cell Types
Figure 3. Membrane proteins were extracted from 5 × 10^6 cells across multiple cell lines using Thermo 89842 and YamayBio BF7475 kits. Protein yield was quantified using a BCA protein assay (e.g., Cat. No. BH5484).
Efficient Membrane Protein Isolation from Tissue Samples
Figure 4. Membrane proteins were isolated from 20 mg of mouse heart tissue using the YamayBio BF7475 protocol. Membrane and cytosolic fractions were analyzed by Western blot using various target antibodies.Related products
Protein Sample Preparation
Protease and Phosphatase Inhibitor Cocktail (100×, EDTA-free)
Product Code: BH5483
Protein Sample Preparation
Protease Inhibitor Cocktail, EDTA-free (100×)
Product Code: BH5481
Protein Sample Preparation
Phosphatase Inhibitor Cocktail (100×)
Product Code: BH5482
Protein Sample Preparation
Ready-to-use BCA Protein Assay Kit
Product Code: BH5484
Protein Sample Preparation
Ready-to-use Bradford Protein Assay Kit (Detergent Compatible)
Product Code: BH5485
Protein Sample Preparation
Lysis Buffer for IP/Co-IP
Product Code: BF7474
Protein Sample Preparation
RIPA Lysis Buffer (Strong)
Product Code: BF7471
Certificate of Analysis
Safety Data Sheet
Specifications
| Product Type | Protein Extraction Kit |
| Form | Liquid |
| Sufficient For | 10 Samples / 50 Samples |
| Reagent Type | Cell Wash Buffer / Cell Permeabilization Buffer / Cell Solubilization Buffer |
| Quantity | 60 mL / 300 mL |
